Intercropped red beet and radish with green bean affected microbial communities and nodulation by indigenous rhizobia

Authors

• Milan Ugrinovic Institute for Vegetable Crops, Karadjordjeva 71, 11420 Smederevska Palanka, Serbia
• Mirjana Mijatovic Institute for Vegetable Crops, Karadjordjeva 71, 11420 Smederevska Palanka, Serbia
• Jasmina Zdravkovic Institute for Vegetable Crops, Karadjordjeva 71, 11420 Smederevska Palanka, Serbia
• Zdenka Girek Institute for Vegetable Crops, Karadjordjeva 71, 11420 Smederevska Palanka, Serbia
• Djordje Kuzmanovic Institute of Soil Sciences, Teodora Drajzera 7, 11000 Belgrade, Serbia
• Natasa Rasulic Institute of Soil Sciences, Teodora Drajzera 7, 11000 Belgrade, Serbia
• Dragana Josic Institute of Soil Sciences, Teodora Drajzera 7, 11000 Belgrade, Serbia

DOI: https://doi.org/10.23986/afsci.41385

Keywords:

Phaseolus vulgaris, Beta vulgaris, Raphanus sativus, PCR, mixed cropping

Abstract

The impact of intercropping green bean (Phaseolus vulgaris L.) with red beet (Beta vulgaris L. var. rubra) and radish (Raphanus sativus L.), two non-legume plants, on the plants’ yields, as well as the effect on occurrence and enumeration of microorganisms in the rhizosphere was studied. The intercrop efficacy evaluation, using Land equivalent ratio, revealed values above 1.0 for all intercropped treatments. Diversity of rhizobia from green bean nodules under different intercropping and fertilizing conditions was observed. On the basis of morphological and biochemical characteristics, 67 out of 158 isolates from green bean roots were selected as rhizobia (42.4%), confirmed by detection of 780 bp nifH gene fragments in nifH-PCR, and then clustered in 27 phenotype patterns. Production of exopolysaccharide succinoglycan was observed in 23 rhizobial isolates, while 6 were detected to solubilize tricalcium phosphate. Screening of genetic diversity using (GTG)₅-PCR fingerprinting showed presence of six different patterns on the 92% similarity level.