PAG electrophoregrams of wheat cultivars grown in Finland

Authors

• Tuula Sontag Department of Food Chemistry and Technology, University of Helsinki, SF-00710 HELSINKI, Finland
• Hannu Salovaara Department of Food Chemistry and Technology, University of Helsinki, SF-00710 HELSINKI, Finland

DOI: https://doi.org/10.23986/afsci.72203

Abstract

The polyacrylamide gel electrophoretic (PAGE) patterns of gliadins of 9 spring wheat cultivars (Apu, Drabant, Taava, Tapio, Ulla, Kadett, Luja, Ruso and Tähti) and of 5 winter wheat cultivars (Aura, Ilves, Linna, Nisu and Vakka) were determined. Most of the samples studied had specific gliadin PAGE patterns, indicating that electrophoregrams obtained with the procedure employed here can be used for identifying wheat cultivars grown in Finland. Only two cultivars, Taava and Ruso, which are close relatives, possessed similar PAGE patterns. The procedure uses a commercial vertical electrophoresis apparatus and thin gels. Up to 28 samples could be electrophoresed in three hours and analyzed after staining. The procedure can be applied in the identification of wheat cultivars currently grown in Finland.