Single seed analysis of fatty acids and glucosinolates combined with meristem rescue in Brassica campestris L.

Authors

• Seppo Sorvari Department of Plant Breeding, Agricultural Research Centre SF-31600 Jokioinen, Finland

DOI: https://doi.org/10.23986/afsci.72283

Abstract

A method for the simultaneous analysis of fatty acids and glucosinolates in a single seed without loosing the original genome was developed. Loss of the original genome was avoided by dissecting the meristem from the seed germinated short time under sterile conditions and by growing an adult plant from it. The rest of the seed was used for fatty acid and glucosinolate analysis by gas chromatography and high-performance liquid chromatography.