Electrofusion of protoplasts of anther-derived dihaploid lines of commercial potato cultivars

Authors

• Veli-Matti Rokka Agricultural Research Centre of Finland, Institute of Crop and Soil Science, Plant Breeding Section, FIN-31600 Jokioinen, Finland
• Yong-Sheng Xu Department of Plant Production, P.O. Box 27, FIN-00014 University of Helsinki, Finland
• Pirjo Tanhuanpää Agricultural Research Centre of Finland, Institute of Crop and Soil Science, FIN-31600 Jokioinen, Finland
• Leena Pietilä Boreal Plant Breeding, Myllytie 10, FIN-31600 Jokioinen, Finland
• Eija Pehu Department of Plant Production, P.O. Box 27, FIN-00014 University of Helsinki, Finland

DOI: https://doi.org/10.23986/afsci.72756

Abstract

Somatic hybrids of anther-derived dihaploid (2x) potato (Solanum tuberosum L.) lines were produced by electrofusion of protoplasts. Using RAPD (randomly amplified polymorphic DNA) markers, six new combinations of dihaploid parental lines from cultivars Matilda, Nicola, Pito, Stina, Van Gogh and White Lady were identified. RAPD marker identification of the putative hybrids was mostly done using two distinct parental line specific primers. 43% of the 76 regenerated calli from the six combinations produced hybrid shoots. Most of the somatic hybrids were tetraploid (4x), but in four fusion combinations plants of hexaploid (6x), octoploid (8x) or mixoploid level were also identified by chromosome counts or flow cytometric nuclear DNA analysis. The mean nuclear DNA content (2C value) of the tetraploid and hexaploid somatic hybrids was lower than the expected DNA content (i.e. the 2C values of the original tetraploid cultivars or the sum of the 2C values of the dihaploid fusion parents). Some somatic hybrids having the expected nuclear DNA content were also found.