DNA markers for Typhula resistance in timothy (Phleum pratense L.)

Authors

• Pirjo Tanhuanpää Green Technology, Natural Resources Institute Finland (Luke), Myllytie 1, FI-31600 Jokioinen, Finland
• Mika Isolahti Boreal Plant Breeding Ltd, Myllytie 10, FI-31600 Jokioinen, Finland
• Nissinen Oiva Formerly at: MTT Agrifood Research Finland, Eteläranta 55, 96300 Rovaniemi, Finland
• Outi Manninen Boreal Plant Breeding Ltd, Myllytie 10, FI-31600 Jokioinen, Finland

DOI: https://doi.org/10.23986/afsci.58477

Keywords:

bulked segregant analysis (BSA), DNA markers, disease resistance, Phleum pratense, timothy, Typhula ishikariensis

Abstract

The objective of the study was to find DNA markers associated with resistance to Typhula ishikariensis in timothy (Phleum pratense L.) using bulked-segregant analysis. A progeny of 161 F1 individuals was created by crossing the Finnish resistant cultivar Tammisto II with the Japanese susceptible cultivar Nosappu. Six to ten clones of each F1 individual were tested for resistance in the greenhouse, and a survival index, which was based both on survival and the ability of plants to recover, was calculated for each F1 to describe resistance. Resistant and susceptible bulks of eight individuals in each were screened with a total of 292 primer combinations. Six DNA markers were found to be associated with resistance, together explaining 15% of phenotypic variation in Typhula resistance. Four of the markers formed one linkage group, which contained a QTL explaining 7% of the variation in Typhula resistance.