Effect of dietary linseed supplements on ù-3 PUFA content and on IGF-1 expression in broiler tissues

Authors

• Zinaida Saprõkina Chemistry, Institute of Veterinary Medicine and Animal Science, Estonian University of Life Sciences, Kreutzwaldi 1A, 51010 Tartu, Estonia
• Avo Karus Chemistry, Institute of Veterinary Medicine and Animal Science, Estonian University of Life Sciences, Kreutzwaldi 1A, 51010 Tartu, Estonia
• Sirje Kuusik Department of Nutrition, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Tartu, Estonia
• Harald Tikk Department of Small Farm Animal and Poultry Husbandry, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Tartu, Estonia
• Peeter Järv Department of Food Sciences, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Tartu, Estonia
• Riina Soidla Department of Food Sciences, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Tartu, Estonia
• Aleksander Lember Department of Small Farm Animal and Poultry Husbandry, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Tartu, Estonia
• Helgi Kaldmäe Department of Nutrition, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Tartu, Estonia
• Virge Karus Chemistry, Institute of Veterinary Medicine and Animal Science, Estonian University of Life Sciences, Kreutzwaldi 1A, 51010 Tartu, Estonia
• Mati Roasto Department of Food Sciences, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Tartu, Estonia

DOI: https://doi.org/10.2137/145960609788066870

Keywords:

broiler, nutrition, linseed, PUFA, IGF-1 expression

Abstract

The aim of this study was to evaluate the effect of ù-3 PUFA-rich linseed-supplemented diet on the ù-6/ù-3 PUFA content and IGF-I mRNA expression in broiler tissues. Broilers (50 of 21 d old and 90 of 42 d old) were divided into groups according to dietary additives: 1.5% linseed oil (LO), 3.0% LO, 15% linseed cake (LC) and 30% LC and to the duration of modified feeding (121, 142 and 2242 days). PUFA content in breast muscles was assayed by gas chromatography and IGF-I mRNA content in liver, muscle and leukocytes was measured by one step RT-PCR. The experimental diets improved the ù-6/ù-3 PUFA ratio through a threefold increase in the ù-3 PUFA content in the breast muscle. The changes in PUFA content were accompanied by changes in IGF-1 gene expression levels in tissues. Therefore, both paracrine and autocrine manner of IGF-1 action are likely to be involved. 15% LC at starting period or 1.5% LO in final diet can be advised as optimal for enriching broiler meat with ù-3 fatty acids.;