Comparative studies about fungal colonization and deoxynivalenol translocation in barley plants inoculated at the base with Fusarium graminearum, Fusarium culmorum and Fusarium pseudograminearum

  • Francesco Pecoraro University of Bologna
  • Marta Giannini University of Bologna
  • Giovanni Beccari University of Perugia http://orcid.org/0000-0002-9227-9023
  • Lorenzo Covarelli University of Perugia
  • Gianfranco Filippini University of Bologna
  • Annamaria Pisi University of Bologna
  • Paola Nipoti University of Bologna
  • Antonio Prodi University of Bologna http://orcid.org/0000-0002-7221-7271
DOI https://doi.org/10.23986/afsci.67704

Abstract

Fusarium crown rot (FCR), an important disease of wheat and barley, is mainly caused by Fusarium graminearum, F. culmorum and F. pseudograminearum, which are also responsible for mycotoxin production. This is the first comparative investigation of their colonization on barley plants after stem base inoculation. At plant maturity, FCR symptoms were visually evaluated, fungal biomass was quantified by Real-Time quantitative PCR and deoxynivalenol (DON) was detected by enzyme-linked immunosorbent assay (ELISA). All the inoculated strains caused the typical FCR necrotic symptoms. Real-Time PCR analysis showed that F. graminearum and F. culmorum were present in the head tissues, while F. pseudograminearum colonized only up to the area including the second node of the stem. Conversely, DON was detected up to the head for all the three species. This study shows that, as already demonstrated in previous research for wheat, DON may be detected up to the head as a consequence of stem base infection by the three FCR agents

Issue
Vol 27 No 1 (2018)
Section
Articles
Published
Mar 30, 2018
How to Cite
Pecoraro, F., Giannini, M., Beccari, G., Covarelli, L., Filippini, G., Pisi, A., Nipoti, P., & Prodi, A. (2018). Comparative studies about fungal colonization and deoxynivalenol translocation in barley plants inoculated at the base with Fusarium graminearum, Fusarium culmorum and Fusarium pseudograminearum. Agricultural and Food Science, 27(1), 74–83. https://doi.org/10.23986/afsci.67704
Authors who publish with Agricultural and Food Science agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Any part of the Agricultural and Food Science may be referred to assuming the Author, The Article, Publication with Volume and Number plus URL for the references have been provided.