ELISA reagents for potato virus Y strains with significantly low non-specific reactions

Authors

• Aarne Kurppa Department of Plant Pathology, University of Helsinki, SF-00710 HELSINKI 71, Finland
• Kirsi Korhonen Labsystems Ltd, Pulttitle 8-11, SF-00810 Helsinki 81, Finland

DOI: https://doi.org/10.23986/afsci.72152

Abstract

High titered and highly virus-specific antisera to selected PVYo and PVYn antigens and their mixture were produced in rabbits. Immunoglobulins purified from the antisera with the protein A method and their enzyme conjugates had very strong virus-specific but no non-specific reactions in the ELISA test. Their homologous reactions to the antigens were stronger than heterologous but any PVY isolate could be identified in a potato leaf sample with the dilutions between 10-2 and 10-3 and in a sample from sprouted tubers with secondary infection diluted between 10-1 and 10-2.