Effect of soil-spraying time on root-colonization ability of antagonistic Streptomyces griseoviridis

Authors

• Hanna Kortemaa Department of Plant Biology, Plant Pathology, PO Box 28, FIN-00014 University of Helsinki, Finland
• Kielo Haahtela Department of Biosciences, Division of General Microbiology, PO Box 56, FIN-00014 University of Helsinki, Finland
• Aino Smolander Vantaa Research Centre, Finnish Forest Research Institute, PO Box 18, FIN-01301 Vantaa, Finland

DOI: https://doi.org/10.23986/afsci.72797

Abstract

The root-colonization ability of Streptomyces griseoviridis Anderson et al. was tested on turnip rape (Brassica rapa subsp. oleifera DC.) and carrot (Daucus carota L.) by the sand-tube method. Nonsterile sand was sprayed with a microbial suspension immediately or 7 days after the seed had been sown. Results expressed as population frequencies and densities indicated that S. griseoviridis effectively colonizes the rhizosphere when the microbe is applied immediately after sowing but less effectively when it is applied 7 days later. Detection values of S. griseoviridis were higher for turnip rape than for carrot. In sterile sand, S. griseoviridis invaribly colonized the rhizosphere of turnip rape after each of the two applications. These findings indicate that S. griseoviridis can compete with indigenous soil microbes in the rhizosphere if it is sufficiently abundant in the soil before the seed emerges. If applied later, however, it competes rather poorly. In root-free nonsterile sand, S. griseoviridis dispersed and survived well.